μBIOSEP Project

Fast and sensitive bioanalysis using micromachined electrospray interfaces with μ-scale separations

Applied research project of Czech-Norwegian consortium introducing miniaturized, highly efficient systems for bioanalyses of challenging samples

 

Project
Goals

Analysis of biomolecules plays a key role in the field of life sciences and the pharmaceutical industry. Since analytes are often present in complex samples, e.g., bodily fluids, hyphenated techniques such as liquid chromatography or capillary electrophoresis with electrospray ionization and mass spectrometry detection are used. Miniaturization generally provides highly efficient and economical analytical tools that are environmentally friendly. The introduction of the following systems thus represents the main goals of the μBIOSEP project:

1.
Micromachined Electrospray Ionization Interfaces

Currently, low flow electrospray ionization interfaces are only supplied by mass spectrometer producers as a part of instruments. The development of versatile electrospray ionization interfaces shall therefore provide platforms for efficient coupling of separation systems with mass spectrometry detection of various manufacturers, suitable for a wide range of applicational settings in the life and pharmaceutical sciences. The concept of the envisaged system is based on the principle of a liquid junction and utilization of very narrow (less than 5 μm) spraying channels characterized by extremely high ionization efficiency and minimum ion suppression.

2.
Miniaturized Recycling Liquid Chromatography System

A device enabling to recycle a sample repeatedly through a single standard analytical column shall provide a virtually unlimited increase of the separation efficiency. The principal concept is based on the Watrex Repetto® system which proved to be applicable on an analytical scale due to the location of the pump outside the separation circuit and minimal void volume of the unit. Under the optimal conditions, it is possible to reach peak efficiency in the order of hundreds of thousands of theoretical plates using the currently available system. Its miniaturization shall further increase the separation efficiency and throughput, and substantially reduce the consumption of chemicals and solvents.

 

Research
Group

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Funding

The µBIOSEP project benefits from a 1.2 mil € grant from Iceland, Liechtenstein and Norway through the EEA Grants and the Technology Agency of the Czech Republic. 

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„Working together for a green, competitive and inclusive Europe”

 

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Roman Řemínek

Principal Investigator

Institute of Analytical Chemistry of the CAS, v. v. i. 

Veveří 967/97

602 00 Brno

Czech Republic

 

Phone: +420 532 290 105

Fax: +420 532 290 182