Currently, low-flow electrospray ionization interfaces are only supplied by mass spectrometer producers as a part of instruments. The development of versatile electrospray ionization interfaces shall therefore provide platforms for efficient coupling of separation systems with mass spectrometry detection of various manufacturers, suitable for a wide range of applicational settings in the life and pharmaceutical sciences. The concept of the envisaged system is based on the principle of a liquid junction and utilization of very narrow (less than 5 μm) spraying channels characterized by extremely high ionization efficiency and minimum ion suppression.

A device enabling to recycle a sample repeatedly through a single standard analytical column shall provide a virtually unlimited increase of the separation efficiency. The principal concept is based on the Watrex Repetto® system which proved to be applicable on an analytical scale due to the location of the pump outside the separation circuit and minimal void volume of the unit. Under the optimal conditions, it is possible to reach peak efficiency in the order of hundreds of thousands of theoretical plates using the currently available system. Its miniaturization shall further increase the separation efficiency and throughput, and substantially reduce the consumption of chemicals and solvents.